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KMID : 0385020020180030120
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Korean Journal of Laboratory Animal Science
2002 Volume.18 No. 3 p.120 ~ p.124
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Prevalence and Pathological Characteristics of Helicobacter spp. in Gastric Mucosa of Domestic Pet Dogs
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Park, Jong-Hwan
Park, Hee-Myung/Seok, Seung-Hyeok/Cho, Sun-a/Lee, Hyi-Yiung/Kim, Dong-Jae/Park, Jae-Hak
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Abstract
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Prevalence of Helicobacter infection in pet dogs and possible existence of positive relations between the presence of Helicobacter spp. and gastritis were studied. Twenty-one dogs, which died of variuos diseased, were referred from three animal hospitals and necropsied. Upon histopathological examination, Helicobacter-like organisms (HLO) were observed only in the gastric mucosa of 5 (23.8%) out of the 21 stomachs. The bacteria mainly colonized in the mucus, gastric pits, the cytoplasm of parietal cells, and the lumen of gastric glands. Through immunohistochemistry using a rabbit polyclonal antibody against H. pylori, positive signals were mainly detected in gastric pits, the lumen of gastric glands, and the cytoplasm of parietal cells, where HLO colonized. In the analysis of PCR with Helicobacter genus-specific primers, 16S rDNA fragment of 374 bp was amplified in the five gastric samples identified with HLO infection through silver staining. The sequence analysis results of each PCR product showed that all had more than 96% homology compared with partial 16S rRNA genes of H. felis, H. heilmannii, H. bizzozeronii, and H. salomonis. Average gastritis scores of the group infected with HLO were 1.250±0.214 and 1.833±0.167 in the fundic and pyloric mucosa, wherease those of uninfected group were 1.000±0.165 and 1.625±0.239, respectively. Accordingly, we concluded that no association exists between HLO infection and gastritis.
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